Leica sp2 confocal microscope manual

Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. May 27, 2015 this feature is not available right now. Leica sp2aobs confocal microscope revised 100108 the leica sp2 confocal microscope is controlled via software lcs leica confocal software. The leica tcssp5 upright confocal laserscanning microscope is. Instructions for the leica sp5 ii laser scanning confocal microscope. Leica aobs sp2 confocal microscopy the leica confocal is one of a few laser scanning confocal microscopes in the aif. Bellow is a brief stepbystep guide to help new users get started smoothly. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack. Beckman institute for advanced science and technology. The leica tcs sp2 upright confocal laserscanning microscope is located in room 170 and can accommodate specimens labeled with nearly all stains except those requiring ultraviolet excitation. Leica sp2 aobs confocal microscope revised 100108 the leica sp2 confocal microscope is controlled via software lcs leica confocal software. The leica tcssp2 upright confocal laserscanning microscope is located in room 170 and can accommodate specimens labeled with nearly all stains except those requiring ultraviolet. Leica sp5 confocal microscope four laser uv, argon, dpss, hene.

Leica sp2 aobs confocal microscope microscopes, imagers and. While the leica tcs sp2 was stateoftheart when purchased, the system no longer meets our needs with respect to multilabel imaging studies, photosensitivity. Reserve the leica sp2 aobs confocal microscope read the user. Image window robarts research institute leica sp5 training manual september 2010. View and download leica sp8 training manual online. The leica tcs is a universal laser scanning confocal microscope system for the biomedical and materials. Enter you name, the date, the time, and the account number in the user log book. Make offer leica microscope dmlb with side by side teaching bridge and. Viewing leica files getting viewing software for windows 6. Switch microscope tft to fluo to activate the incident path from default cs in combi.

This system has five lasers generating nine lines for fluorescence excitation 405, 458, 476, 488, 496, 514, 543, 594, 633 nm. Leica sp2 confocal the uv aotf cooling fan bearing is failing. Please contact your sales person with possible substitutions. Researchers have the option of using three different lasers an argon, krypton, and a heliumneon laser to excite fluorescently. Home in vitro imaging leica tcs sp2 aobs spectral confocal leica tcs sp2 aobs spectral confocal manuals. The sp2 is a sensitive, flexible confocal for imaging fixed samples. Leica tcssp5 upright confocal laserscanning microscope. In a point scanning confocal system, the microscope lenses focus the laser light on one point in the specimen at a time the. Confocal microscopy detects structures by collecting light from a single focal plane of. This system has five lasers generating nine lines for fluorescence. No problem the leica tcs spe adds a confocal to your prepared fluorescence microscope. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. The leica tcs sp ii system makes it possible to image a single, infocus plane horizontal or vertical as well as a series of planes.

The system is equipped with an inverted microscope encased with an incubator for livecell imaging. The advantage of having a stack of serial optical sections. Combine two worlds the leica tcs spe is based on our high end widefield. Trained users are issued an account on the online booking calendar 0. Leica tcs sp2 laser scanning spectral confocal microscope part 1. Manual for leica sp2 confocal microscope enter you name, the date, the time, and the account number in the user log book.

Jan 11, 2008 basic training video for use of the leica confocal microscope at tufts. Confocal microscopy facility schepens eye research. Use windex and cotton balls or kimwipes to clean coverslips. Zeiss axio observer z1 manual single or tile imaging with zen. The leica sp2 confocal microscope is controlled via software lcs leica. Superior image performance, all the configurable as a personal, multiuser or advanced imaging center. Transmitted light detector and manual condenser with dic optics. Leica sp8 upright confocal duke light microscopy core. Reserve the leica sp2 aobs confocal microscope read the user manual. Training manual leica tcs sp5 confocal microscope september 2010. Please browse the internet to learn interactively all about. Leica dm6000cs stand upright motorized scanning stage lasers.

Two of these detectors are very sensitive hyd sp gaaspdetectors. Also see zeiss lsm780, leica sp8, nikon spinning disk confocal, api omx thin samples, tissue culture and sp5 2photon thick tissue for more live cell imaging options. The zmb owns an inverted leica tcs, a tsc sp2 confocal system with upright and inverted microscope stand and an inverted. The existing multiuser confocal system, a leica tcs sp2 purchased in 2002 has been in continuous operation for over a decade and provided outstanding service to the research community. Check your specimen under a conventional fluorescent microscope and confirm the staining. This manual is the first section of a three part leica tcs sp5 user guide edited by donald pottle. The leica software will startup and you will see the leica confocal software window. The confocal microscope system for highest demands in multiuser facilities.

A single vertical section, or xzscan, allows you to see your sample as though from the side. The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed specimens. Combine two worlds the leica tcs spe is based on our high end widefield fluorescence microscopes. Basic training video for use of the leica confocal microscope at tufts. You choose the platform on which you build the confocal functionality. Ultimate optical design and stability with extreme sensitivity and superb image quality. Introduction to confocal laser scanning microscopy leica.

Dealing with software crashes and microscope stand lockups. Leica tcs 4d first fourdetector confocal for multidye applications first spectral detector in confocal microscopy leica tcs sp2 first confocal with excitation from uv to ir hc optics system ensures superlative optical performance first use of aotf for laser modulation in a confocal first dedicated objectives for confocal scanning cs. Make sure that your sample slides are clean and sealed. Leica tcs sp5 confocal user manual only people who have had an introduction from kees straatman are allowed to operate the system. Enter you name, the date, the time, and the account number. Many pages are copied from internet web pages or from presentations given by leica, zeiss and other companies.

The following is the details for confocal platform as well as two microscopes. Leica sp5 inverted confocal manual duke light microscopy. Fan on turn laser power level to lowest setting min turn argon laser key to start briefly, and then back to on turn green and red hene lasers on with keys if needed. Leica sp5 laser scanning confocal microscope ucsf drc. Leica tcs sp5 confocal laser scanning microscope user guide. Introduction to confocal laser scanning microscopy leica this presentation has been put together as a common effort of urs ziegler, anne greet bittermann, mathias hoechli. Leica am total internal reflection fluorescence manual. The existing multiuser confocal system, a leica tcs sp2 purchased in 2002 has been in continuous operation for over a decade and provided outstanding service to the research.

This leica sp2 platform is equipped with two leica confocal microscopes. The laser light emitted from the leica tcs sp2 used in accordance with. White light laser system 470 to 670 nm tunable in steps of 1 nm. The leica may be programmed to scan many different wavelength. Superior image performance, all the configurable as a personal, multiuser or advanced imaging center multiple innovations from. The leica may be programmed to scan many different wavelength ranges. Confocal microscopes confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in threedimensional imaging and exact examination of subcellular structures and dynamic processes. Leica sp2 aobs confocal microscope microscopes, imagers. The leica tcs sp2 system was designed for confocal recording and. Confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in three.

Scanning as well as controlling the motorized leica dm ire2 microscope. Conducting a crosstalk test using the leica tcs sp5 confocal microscope. The leica tcs is a universal laser scanning confocal microscope system for the biomedical and materials research environment. The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living. With the use of the multiphoton laser the microscope system becomes inherently confocal. Raise stage to appropriate height and mount sample. To always keep the information up to date, the description of software functions was intentionally omitted from these operating instructions. The leica tcs sp2 is supplied with the latest version of the licensed leica confocal software. Leica microscope dmlb with side by side teaching bridge and. Leica sp5 training manual september 2010 for the argon laser, adjust the slider to 2030%.

An introduction from your bosssupervisor is not enough the system has 5 detectors. The leica confocal microscope system b46 weill hall revised65 startup turn on 3 red buttons on far right. Leica sp8 upright confocal duke light microscopy core facility. Leica tcs 4d first fourdetector confocal for multidye applications first spectral detector in confocal microscopy leica tcs sp2 first confocal with excitation from uv to ir hc optics. This laser based system is ideal for fixed photostable applications where high confocal resolution is critical. Starting the lcs software on the leica tcs sp5 confocal microscope.

What is a confocal microscope webinar leica microsystems. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix. Instructions for leica sp2 confocal equipped with visible. Leica tcs sp8 confocal microscope system andrew lackner. Leica tcs sp2 confocal microscopes products home leica leica microsystems.